Initially, you may notice some symptoms of staph infection. You may see a little bump on the skin that looks infected - it might be red and swollen with pus. MRSA infections are commonly initially mistaken for a spider bite. You may have a fever as well - this is when it's time to call your health care provider right away.
A MRSA infection could also become quite severe, especially if it spreads to the bloodstream, or other areas like the heart and lungs (this is more common in those who are already hospitalized). Symptoms of this infection include chest pain, cough or shortness of breath, fatigue, fever and chills, general ill feeling, headache, rash, and wounds that don't heal.
Once infection is suspected, the actual diagnosis is made in the lab. If you have a wound, a health care professional will swab it and take the sample to the lab. The lab will take the sample, place it into a solution, and look for Gram-positive cocci clusters (this describes the color and shape of the stained bacteria).
The sample is tested for Staphylococcus aureus as well. This is done by placing it in a tube with plasma from rabbit's blood and free coagulase. If they find that a clump has formed, this indicates that staph is present.
The next test is to see if this type of bacteria is resistant to medicine. If it continues to grow despite antibiotics, then this is how it is confirmed to be MRSA.
There are also several other ways of obtaining samples to test for MRSA. These include sputum cultures - this is when you simply cough up sputum that can be tested. Urine cultures are also used quite frequently. This could be collected from a foley catheter, or with a cup. If a blood culture is tested and returns positive for MRSA, this would diagnose a bloodstream infection.
Sources:
http://www.cdc.gov/mrsa/pdf/MRSA_Broch_Parent.pdf
http://www.nytimes.com/health/guides/disease/mrsa-infection/overview.html
http://www.wikihow.com/Test-for-MRSA
http://www.healthline.com/health/mrsa#Diagnosis6
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